Untitled Document
REDLARA associated centers
 
CLINICAL EMBRYOLOGY COURSE
 
 Clinical Embryologist Course 

Course for clinical embryologist offered by the Latin American Network of Assisted Reproduction

Objectives:
  I – Update the theoretical formation of the clinical embryologists of the RED or other interested professionals in this area.
  II – Discuss, update and interchange practical information.  

MODULE I
        Basic and Clinical Aspects for the Embryologist
        Duration: Theoretical: 4 months
        Practical: 1 month

I. Female Gametes
II. Male Gametes
III. Fertilization, zygote formation and embryonary development.
IV. Clinical aspects of the reproductive function and infertility

MODULE II
        Laboratorial fundamentals of the fertilization process, culture and micromanipulation of gametes and embryos
        Duration: Theoretical: 3 months
        Practical: 1 month

I. Assisted Reproduction Laboratory Organization
II. Embryo culture and tissues

MODULE III
        Micro-manipulation of gametes and embryos
        Duration: Theoretical: 2 months
        Practical: 2 months

I. Micromanipulation and assisted reproduction
II. Manipulation and its use in ART

MODULE IV
        Gamete and embryo cryopreservation
        Duration: Theoretical: 2 months
        Practical: 15 days

I. Principles of Cryobiology
II. Gamete cryopreservation
III. Embryo cryopreservation
IV. Gonadal tissue cryopreservation

MODULE V
        Genetics and Bio-ethic for the clinical embryologist
        Duration: Theoretical: 2 months

   

I. Genetic in infertility
II. Ethic and legislation in Reproductive Biology


 
 
 
 

I. Female Gametes
  a. Anatomy of the female reproductive function
  b. Oogenesis
  Basic Endocrinology of the oogenesis
  Principles of Meiosis (arrest and release of meiosis)
  Growth and development of human oocytes
  Follicular development
  c. Functional Marking: biochemistry, genetic and molecular
  d. Collection, identification and preparation of oocytes for IVF/ICSI
  e. Culture and oocyte maturation
  f. External factors that may affect the oocyte quality
  g. Normal oocyte features
  h. Endocrinologycal repercussions in the ovarian working alterations
  i. Ovarian patology
 
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II. Male Gametes
  a. Anatomy of the male reproductive function

b. Spermatogenesis
  Basic endocrinology of the spermatogenesis
  Principles of meiosis
  Growth and development of human spermatozoa

c. Semen composition
  Biochemistry and physiology of the semen

d. Semen laboratory assessment
  Semen collection
  Instruments for the semen assessment
  Counting chambers
  Electronic gadget
  Macroscopic measures
  Sperm motility
  Sperm concentration
  Sperm morphology
  - Classical criteria
  - Kruger strict criteria

e. Additional studies for male factor evaluation
  Detection of sperm antibodies
  Hypo-osmotic swelling test (HOS)
  Sperm recovery test
  Sperm survival test
  Chromatine test
  Acrossomal test
  Sperm-culture, determination of Chlamydia and Mycoplasma

f. Standard normal semen according to WHO

g. Semen preparation:
  Basic techniques for sperm recuperation
  - Standard swim-up or layering
  - Density gradients
  Spermatozoa from ejaculate
  Retrogade ejaculation and electroejaculation
  Epididymal sperm
  Testicular sperm
  Immature spermatozoa
  Semen for IUI/IVF/ICSI

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III. Fertilization, zygote formation and embryonary development.

III.1. Fertilization:
  a. Sperm-oocyte interaction
  b. Spermatozoa activation
  c. Oocyte activation
  d. Cortical reaction
  e. Fusion

III.2. Zygote formation
  f. Pronucleous formation
  h. Syngamy

III.3. Embryonic development and implantation
  i. First stage of the development
  j. Zygote genome activation
  k. Imprinting
  l. Compaction
  m. Blastocyst formation
  n. Embryo implantation

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IV. Clinical aspects of the reproductive function and infertility

IV.1. Female reproductive function
  a. Infertile woman investigation
  - General examination
  - Infertility signs
  - Normal ovulation outline
  - Ovulation evaluation
  - Ultrasonografic evaluation
  - Endocrinological evaluation

b. Non-ovulatory infertility
  - Diagnosis
  - Causes
  - Regimen of ovarian stimulation

c. Protocols of ovarian stimulation for artificial insemination
  In vitro fertilization and ICSI


  IV.2. Male reproductive function
  a. Infertile man investigation
  - General examination
  - Infertility signs
  - Normal semen parameter (According to WHO)
  - Sperm analysis interpretation
  - Sperm patology
  - Endocrinological evaluation
  - Endocrinological repercussions of the male gametogenesis alterations

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I

I. Assisted Reproduction Laboratory Organization
   
  a. Basic equipment
  Incubators
  Filters in the laboratory
  Flow hoods
  Centrifuges
  Osmometers
  Freezing machine
  Liquid Nitrogen storage Dewars
  Microscopes
  Micromanipulators
  Electrical appliances
  Importance of filters in incubators and in the laboratory
  Different types of filters: advantages and disadvantages

b. Quality control
  b.1: Microbiology and ART
  Infectious diseases and ART
  Gamete donation and disease transmission
  Infection control and disease transmission
  Sterilization and Disinfection (Chemical and physical agents)

b.2: Material supply and culture media

b.3: Quality control system
  Equipment control and environment
  Procedure control (standard manuals)
  Bioassays
  Health and safety in the IVF laboratory

c – Rules for accrediting a laboratory in RED LARA

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II. Embryo culture and tissues
   
  a. Oocyte collection
  - Protocols for ovarian stimulation
  - Oocyte identification and classification

b. Insemination
  - IVF
  - ICSI

c. Fertilization assessment

d. Embryo culture
  - Culture media
  - Protein supplements
  - Culture under oil
  - Tissue culture systems

e. Embryo grading
  - Pronuclei
  - Early cleavage
  - Cleaved embryos
  - Blastocyst

d. Embryo transfer
  - Embryo selection criteria

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I. Micromanipulation and assisted reproduction
   
  a. Micromanipulation techniques:
  - PZD
  - SUZI
  - ICSI
  - Assisted Hatching
  - Embryo Biopsy

b. Equipment for micromanipulation:
  - Micropipettes
  - Micromanipulators, microscopes and others

c. Micromanipulation Protocols
  - Equipment preparation
  - Protocols

d. ICSI Indications
  - Sperm source
• Ejaculate
• Epididymus
• Testis

e. Risk and inheritance after ICSI

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II. Manipulation and its use in ART
   
  a. Multi potent stem cells
  - types of cells

b. Embryonic stem cells
  - generation
  - application

c. Cloning
  - cloning methodology
• reproductive cloning
• non-reproductive cloning (therapeutic)
  - problems with cloning

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I. Principles of Cryobiology
   
  a. Effects of reduced temperature
  b. Ice formation.
  c. Cryoprotectors
  d. Factors that affect the freezing
  - Cooling rate
  - Seeding
  - Removal of cryoprotectant
  e. Freezing protocols
  - slow
  - rapid
  - ultra-rapid (vitrification)
  f. Thawing protocols
  g. Storage and safety in freezing samples
 
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II. Gamete cryopreservation
   
  II.1. Oocytes
  a. Principles of oocyte cryopreservation
  b. Effects of cryopreservation of oocytes
  c. Immature oocyte freezing
  d. Freezing protocols
  e. Thawing protocols

II.2. Spermatozoa
  a. Effects of cryopreservation of sperm
  b. Freezing protocols
  c. Epididymal and testicular sperm freezing
  d. Methods to improve survival

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III. Embryo cryopreservation.
   
  a) Freezing in different embryo stages
  b) Cryoprotectors
  c) Embryo transfer
  d) Cryopreservation program benefits
  e) Freezing protocols
  f) Thawing protocols

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IV. Gonadal tissue cryopreservation
   
  IV.1. Ovarian tissue
  a. Follicles cryopreservation and survival
  b. Grafting strategies
  c. Freezing protocols
  d. Current status of the freezing results

IV.2. Testis tissue
  a. Semen cryopreservation of patients with cancer
  b. Grafting strategies
  c. Freezing protocols
  d. Current status of the freezing results

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I. Genetic in infertility
   
  a. Gene expression
  b. Structural and numeric chromosomal abnormalities
  c. Genetic disorder
  - Down Syndrome
  - Cystic Fibrosis
  - Others
  d. Preimplantation diagnosis
  - Material selection for PGD
  - polar body and blastomere Biopsy
  - FISH - PCR

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II. Ethic and legislation in Reproductive Biology


  a. Medicine and ethics
  b. Bio-ethics theory
  c. Bio-ethics principle
  d. Bio-ethics position in relation to:
  - Respect to the human being and the embryo manipulation
  - Gamete and embryo donation
  - Freezing embryos
  - Sex selection
  - Cloning
  - Research with human embryos
  - Surrogacy
  - Genetic counselling
  e. Laws in Assisted Reproduction Techniques
  - Latin America / USA / Europe

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In this section you´ll find information about the accredited and affiliated centres that belong to the Latin American Network of Assisted Reproduction.